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Title   혈관생성 및 관상구조 형성에 미치는 히스타민의 영향 ( The Role of Histamine on Angiogenesis and Capillary Network Formation )
Publicationinfo   1993 Jan; 025(04): 607-616.
Key_word   Angiogenesis, Histamine, Cimetidine
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Abstract   Angiogenesis is a prerequisite for the development of various vascular diseases and for the growth and metastasie of solid tumors. In this study, the effects of histamine on angiogenesis were examined ueing an in vitro caculture system of rat epididymal fat myofibroblasts and microvascular fragments. Histamine induced prominent capillary network formation without affecting the proliferation of myofibroblasts. To determine the mechanism of histamine action on angiogenesis the changes in cell numbers, DNA synthesis, phosphorylation of ribosomal protein S6, and cAMP content were assessed before and after the treatments of histamine-receptor antagonists. The ability of bistamine to stimulate capillary network formation was blocked by pyrilamine, a H - receptor antagonist, reflecting histamine acts through the H receptors in the capillary endothelium. Cimetidine, a H,-receptor antagonist, itself demonstrated five fold increase of myofibroblasts proliferation along with moderate enhancement of capillary network formation. Our results suggest that cimetidine works through the reduction of cAMP content in myofibroblasts, thereby releasing the inhibitory effect of cAMP on myofibroblasts. Thus, myofibroblasts pro- liferation induced by cimetidine stimulates the production of angiogenic factors from myofibroblasts leading to indirect enhancement of capillary network formation.
저 자   김인순(In Soon Kim),이석용(Seok Yong Lee),김정회(Jeong Hoe Kim),조영진(Young Jin Cho),이상복(Sang Bok Lee)